ANSWERS TO LAB REVIEW

1. See "Water will move" handout

DISTILLED WATER

20% sugar solution

LOW OSMOTIC CONCENTRATION

HIGH OSMOTIC CONCENTRATION

HYPOTONIC
Few dissolved particles

HYPERTONIC
Many dissolved particles

HIGH FREE ENERGY (orderly)

LOW FREE ENERGY (more disorder)

HIGH OSMOTIC POTENTIAL

LOW OSMOTIC POTENTIAL

Low MOLARITY (M)

High MOLARITY (M)

Low ENTROPY (orderly)

High ENTROPY(more disorder)

LOW OSMOTIC PRESSURE
(Tendency of water to move in is low)

HIGH OSMOTIC  PRESSURE
(Tendency of water to move in is high)

HIGH WATER POTENTIAL (ψ)

LOW WATER POTENTIAL (ψ)

2. Tube 3 (0.2 M inside:0.2M outside) will change the least

3. catalase is the enzyme; hydrogen peroxide is the substrate

4. acid denatures the enzyme

5. D mitosis divides once; meiosis divides twice

6..telophase is called reverse prophase

7. Rf = __Distance substance moved_ = 11.5/12 = 0.96
              Distance solvent front moved

8. C-DPIP accepts electrons

9. .8 ml/20 min = 0.04 ml/min

10. D-KOH binds CO2  and removes it

11. Plate II -plasmid + antibiotic

12. LANE 3- BamHI cuts into 3 fragments; 2kb, 6kb, 12kb

13. D- genes for eye color and wings are inherited independently
No difference in males:females; See 9:3:3:1 ratio;
98 red/normal; 22 red/no wings; 26 sepia/normal wings; 14 sepia/no wings

14.   X2 = 5.14  Look on table. 1 degree of freedom/0.05 column = 3.841     5.14 > 3.841 so reject the hypothesis that there is no difference between observed and expected.

15. 1/10,000 = q2
    So q = 0.01
    p + q = 1 so p = 0.99
  Heterozygous = 2pq = 2 (.99) (0.1)= 0.02 or 2%

16. D. frequency of dominant or recessive alleles is not one of the conditions for Hardy-Weinberg equilibrium to exist

17.  Warm, breezy will transpire more

18. D-water moves from high water potential to low water potential: spongy mesophyll in leaves will have the lowest water potential

19. increasing temperature increases heart rate in daphnia

20. snake will be most affected by changes in environment; fish is cold blooded too but lake won't change temperature as rapidly

21. taxis is directional (toward or away): kinesis in increased movement but random

22. E- there is a preference.

23. screens simulate light reaching different depths in the lake

24. Net productivity with decrease (more respiration compared to photosynthesis) due to less light getting through to the bottom