RESPIRATION THEATER

THINGS YOU WILL NEED:
Roll of Velcro with adhesive on back
yarn
hole puncher

AHEAD OF TIME
1. Copy off the role play cards onto cardstock OR colored paper and laminate them.
2. Copy the C and CO₂ cards back to back
Copy the CoEnzyme A and Acetyl Co-A cards back to back
Copy the card with 4 C's and the CITRIC ACID card back to back
3. Cut apart the e^-, P, and H⁺  cards.

4. Use a paper punch to put holes in the following cards and attach string so card can be worn around the neck. (ATP synthase, Matrix, Intermembrane space, ETC,  NAD+, Adenosine P,  C/CO2, Krebs cycle)

5. Cut small strips of Velcro at attach the hook side to the front of to Adenosine P P card and the loop side to the back of the P circle so it can be attached to make ATP. Cut the "hook" strip about 1/2 the size of the "loop" side. Otherwise they grab too much and rip off easily. 6. Do the same with the NAD+  and the H+ cards.

7. Do the same with the FAD card to make places to attach 2 H+ to make a FADH2 molecule.

8. Do the same with the O card to make places to attach 2 H+ to make a H2O molecule.

MINIMUM # OF CARDS YOU WILL NEED:

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ACT IT OUT:ELECTRON TRANSPORT:

Students wear appropriate name tags and line up in correct position.
ETC's and ATP synthase stand in a line ( I use 3 for Biology. 5 for AP and talk about it)
2 Matrix people stand in front of line.(This is a busy job so you can add a 3rd if you want)
2 Intermembrane space people stand behind line.
ADP stands near ATP synthase
NADH and FADH₂ people stand near front of ETC line
ADP people stand near ATP synthase

Roles: 
NADH: Passes 2 e^- cards to first ETC, gives H⁺ to Matrix.
FADH₂ passes 2 e^- cards to SECOND ETC, gives H⁺ to Matrix
ETC: ALL pass e^- cards along line if you use 3 ETC pump H⁺ cards over shoulder to intermembrane space;
(If you use 3 ETC-ALL pump H+; if you use 5 ETC -1st, 3rd, and 5th ETC pump)
Last ETC passes electrons to O
O: Receives 2 e- cards from last ETC and  2 H⁺ from matrix to become water
MATRIX: Receives H⁺ from NADH & FADH₂; passes H+ to ETC; receives H+ from ATP SYNTHASE
INTERMEMBRANE SPACE:  Receives H⁺ cards from ETC and passes H⁺ cards to ATP SYNTHASE
ATP SYNTHASE:  receives H⁺ cards from intermembrane space and passes them to matrix; holds P cards and attaches them to ADP.

Walk through everyone's job first. Compare to ETC in light dependent reactions.
Where is this happening? What are the jobs? Where do electrons come from? Where is ATP and WATER made? Where does H⁺  build up? How many H+ are pumped from NADH? From FADH₂?
Where does NAD₊ go once it has dropped its electrons?
Then let them do it on their own. Then do it without you telling them what to do.
Then trade jobs with someone and do it again.

LATER CAN ADD IN: GLYCOLYSIS,  ACETYL CO-A charging OR KREBS CYCLE IF DESIRED

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GLYCOLYSIS

6 C people each hold 2 e- cards and link arms to represent a 6 carbon glucose
GLYCOLYSIS: receives a P from 2 ATP's; breaks 6 C's into TWO 3-C molecules
3 C group (pyruvate): gives e- cards to NAD⁺
CYTOPLASM: Gives NAD⁺ a H⁺
2 NAD⁺ people: Each receives 2e- from 3C group and H⁺ from CYTOPLASM
GLYCOLYSIS:Gives a P each to 4 ADP
ADP: receives a P from glycolysis
3 C group (pyruvate): goes on to ACETYL CO-A charging

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ACETYL CO-A Charging:

3 C people each hold 2 e- cards and link arms to represent a 3 carbon pyruvate
Last C in line is released and gives its e- cards to NAD⁺ and flips their card to show CO₂ side.
MATRIX: Gives H⁺ to NAD⁺
Remaining 2 C's join with COENZYME A and COENZYME A flips their card over to show ACETYL CO-A side
ACETYL CO-A carries C's to KREBS CYCLE; flips their card back to COENZYME-A side and returns to ACETYL CO-A CHARGING to find another 3 C pyruvate

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KREBS CYCLE:
4 Krebs people stand in ring (Krebs #1 person has 4 /citric acid card)
Krebs people move in a circle when cycling.
Krebs #1 (4C/citric acid) person:  receives 2C's from Acetyl Co-A and welcomes them into circle
Breaks off a C from and receives e- cards from C and passes them to NAD⁺
Krebs #2 person: Breaks off one of C's that entered, takes their 2e- cards and passes them to NAD⁺
MATRIX: Gives H⁺ to NAD⁺
Krebs #3 person: Breaks off last of C's that entered, takes their 2e- cards and passes them to FAD
MATRIX: Gives H⁺ to FAD
Krebs #4 gives P to ADP to make ATP
C: passes E- cards to Krebs cycle, flips card to CO₂ side, and leaves the circle
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